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Biotechnology Information computer program blastp
Computer Program Blastp, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer+software+program/blastp/us12617826-81-14-25
Average 86 stars, based on 1 article reviews
computer program blastp - by Bioz Stars, 2026-09
86/100 stars

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Amplification:

Article Title: Novel aflatoxin oxidase CotA alleviates aflatoxin B 1 toxicity in broilers by reducing hepatic AFB 1 residues and inhibiting oxidative stress-mediated ferroptosis.
Article Snippet: Quantitative real-time PCR (qRT-PCR) was carried out on a Bio-Rad CFX Opus 96 Real-Time PCR System using the ChamQ Universal SYBR qPCR Master Mix (Cat# Q711-02, Vazyme Biotechnology, Nanjing, China). .. The thermal cycling protocol comprised an initial pre-denaturation at 95◦C for 30 s, followed by 45 cycles of three-step amplification: 95◦C for 5 s, 60◦C for 30 s. Specific primers for target genes and the housekeeping gene (GAPDH) were designed using Primer 5.0 software (National Center for Biotechnology Information, Bethesda, MD, USA). .. The primer sequences, listed in Table 2, were synthesized by Sangon Biotech (Shanghai) Co., Ltd.

Article Title: Novel aflatoxin oxidase CotA alleviates aflatoxin B 1 toxicity in broilers by reducing hepatic AFB 1 residues and inhibiting oxidative stress-mediated ferroptosis
Article Snippet: Quantitative real-time PCR (qRT-PCR) was carried out on a Bio-Rad CFX Opus 96 Real-Time PCR System using the ChamQ Universal SYBR qPCR Master Mix (Cat# Q711-02, Vazyme Biotechnology, Nanjing, China). .. The thermal cycling protocol comprised an initial pre-denaturation at 95°C for 30 s, followed by 45 cycles of three-step amplification: 95°C for 5 s, 60°C for 30 s. Specific primers for target genes and the housekeeping gene (GAPDH) were designed using Primer 5.0 software (National Center for Biotechnology Information, Bethesda, MD, USA). .. The primer sequences, listed in , were synthesized by Sangon Biotech (Shanghai) Co., Ltd.

Software:

Article Title: Novel aflatoxin oxidase CotA alleviates aflatoxin B 1 toxicity in broilers by reducing hepatic AFB 1 residues and inhibiting oxidative stress-mediated ferroptosis.
Article Snippet: Quantitative real-time PCR (qRT-PCR) was carried out on a Bio-Rad CFX Opus 96 Real-Time PCR System using the ChamQ Universal SYBR qPCR Master Mix (Cat# Q711-02, Vazyme Biotechnology, Nanjing, China). .. The thermal cycling protocol comprised an initial pre-denaturation at 95◦C for 30 s, followed by 45 cycles of three-step amplification: 95◦C for 5 s, 60◦C for 30 s. Specific primers for target genes and the housekeeping gene (GAPDH) were designed using Primer 5.0 software (National Center for Biotechnology Information, Bethesda, MD, USA). .. The primer sequences, listed in Table 2, were synthesized by Sangon Biotech (Shanghai) Co., Ltd.

Article Title: Bacteriophages for the control of bacterial speck disease
Article Snippet: .. Percent identity can be determined using any homology comparison software, including for example, the BLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters. ..

Article Title: Novel aflatoxin oxidase CotA alleviates aflatoxin B 1 toxicity in broilers by reducing hepatic AFB 1 residues and inhibiting oxidative stress-mediated ferroptosis
Article Snippet: Quantitative real-time PCR (qRT-PCR) was carried out on a Bio-Rad CFX Opus 96 Real-Time PCR System using the ChamQ Universal SYBR qPCR Master Mix (Cat# Q711-02, Vazyme Biotechnology, Nanjing, China). .. The thermal cycling protocol comprised an initial pre-denaturation at 95°C for 30 s, followed by 45 cycles of three-step amplification: 95°C for 5 s, 60°C for 30 s. Specific primers for target genes and the housekeeping gene (GAPDH) were designed using Primer 5.0 software (National Center for Biotechnology Information, Bethesda, MD, USA). .. The primer sequences, listed in , were synthesized by Sangon Biotech (Shanghai) Co., Ltd.

Article Title: Comparative physiology and cellular responses of different peanut cultivars under PEG-induced drought at seedling stage.
Article Snippet: .. Gene coding sequences (CDS sequences) were identified at the National Center of Biotechnology Information (NCBI), and gene primers were designed using Primer Premier 5.0 software. ..

Article Title: Single-Cell Sequencing Analysis-Driven Nanomedicine Design for Mimicking Endogenous Immune Repair in Myocardial Infarction.
Article Snippet: Mimicking endogenous immune cell responses holds great promise for repairing tissue damage in myocardial infarction (MI), yet challenges persist due to the heterogeneity, spatiotemporal dynamics, and diverse functions of macrophage subtypes during post-MI repair.. Herein, we introduce a data-driven biomimetic strategy that leverages the sequential delivery of immune cell-mimicking nanoparticles to replicate the natural immune repair mechanisms.. Using single-cell RNA sequencing, we analyzed the dynamic changes in immune cells during post-MI repair, revealing the distinct roles of macrophage subtypes in different stages of recovery.

Article Title: Genomic and secondary metabolites of the marine cyanobacterium Capilliphycus salinus ALCB114379.
Article Snippet: .. To construct the phylogenetic tree based on the 16S rRNA gene, 130 sequences were selected from the National Center for Biotechnology Information (NCBI) and aligned using MUSCLE (Edgar, 2004) with the MEGA 7.0.26 software. ..

Comparison:

Article Title: Bacteriophages for the control of bacterial speck disease
Article Snippet: .. Percent identity can be determined using any homology comparison software, including for example, the BLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters. ..

Article Title: CLEC2 fusion protein and uses thereof
Article Snippet: .. For purposes herein, sequence comparison between two amino acid sequences was carried out by computer program Blastp (protein-protein BLAST) provided online by Nation Center for Biotechnology Information (NCBI). ..

Article Title: Compositions and uses thereof in treating cancers
Article Snippet: .. For purposes herein, sequence comparison between two nucleotide sequences was carried out by computer program BLASTN (nucleotide-nucleotide BLAST) provided online by Nation Center for Biotechnology Information (NCBI). ..

Sequencing:

Article Title: CLEC2 fusion protein and uses thereof
Article Snippet: .. For purposes herein, sequence comparison between two amino acid sequences was carried out by computer program Blastp (protein-protein BLAST) provided online by Nation Center for Biotechnology Information (NCBI). ..

Article Title: Compositions and uses thereof in treating cancers
Article Snippet: .. For purposes herein, sequence comparison between two nucleotide sequences was carried out by computer program BLASTN (nucleotide-nucleotide BLAST) provided online by Nation Center for Biotechnology Information (NCBI). ..

Gene Expression:

Article Title: Single-Cell Sequencing Analysis-Driven Nanomedicine Design for Mimicking Endogenous Immune Repair in Myocardial Infarction.
Article Snippet: Mimicking endogenous immune cell responses holds great promise for repairing tissue damage in myocardial infarction (MI), yet challenges persist due to the heterogeneity, spatiotemporal dynamics, and diverse functions of macrophage subtypes during post-MI repair.. Herein, we introduce a data-driven biomimetic strategy that leverages the sequential delivery of immune cell-mimicking nanoparticles to replicate the natural immune repair mechanisms.. Using single-cell RNA sequencing, we analyzed the dynamic changes in immune cells during post-MI repair, revealing the distinct roles of macrophage subtypes in different stages of recovery.

Construct:

Article Title: Genomic and secondary metabolites of the marine cyanobacterium Capilliphycus salinus ALCB114379.
Article Snippet: .. To construct the phylogenetic tree based on the 16S rRNA gene, 130 sequences were selected from the National Center for Biotechnology Information (NCBI) and aligned using MUSCLE (Edgar, 2004) with the MEGA 7.0.26 software. ..



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